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18S-rDNA SEQUENCING, ENZYME PATTERNS AND MORPHOLOGICAL CHARACTERIZATION OF TRICHOPHYTON ISOLATES BJM
Nascimento,Adriana Mendes do; Martinez-Rossi,Nilce M..
Dermatophytes, capable to use keratin of the host for nutrition, belong to one of the major groups of pathogenic fungi. Since dermatophytes are a closely related group they share various common features, and the morphology of isolates of a given species can be atypical, making species identification and differentiation even more difficult. Many methods have been explored in attempts to distinguish dermatophytes, but the combined use of different approaches for the investigation of the intraspecific and interspecific variability of Trichophyton continues to be scarce. Some studies have shown that amplified fragments of the small ribosomal DNA subunit 18S contains variable regions which can be used to discriminate between medically relevant yeast species,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trichophyton; 18S-rDNA sequencing; Extracellular enzymes; Dermatophyte.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300004
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Antifungal activity of extracts from Piper aduncum leaves prepared by different solvents and extraction techniques against dermatophytes Trichophyton rubrum and Trichophyton interdigitale BJM
Santos,Maximillan Leite; Magalhães,Chaiana Froés; Rosa,Marcelo Barcellos da; Santos,Daniel de Assis; Brasileiro,Beatriz Gonçalves; Carvalho,Leandro Machado de; Silva,Marcelo Barreto da; Zani,Carlos Leomar; Siqueira,Ezequias Pessoa de; Peres,Rodrigo Loreto; Andrade,Anderson Assunção.
The effects of different solvents and extraction techniques upon the phytochemical profile and anti-Trichophyton activity of extracts from Piper aduncum leaves were evaluated. Extract done by maceration method with ethanol has higher content of sesquiterpenes and antifungal activity. This extract may be useful as an alternative treatment for dermatophytosis.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antifungal activity; Piper aduncum L.; Dermatophytes; Trichophyton; Extraction techniques.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400035
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Dermatophytosis diagnosed at the Evandro Chagas Institute, Pará, Brazil BJM
Silveira-Gomes,Fabíola; Oliveira,Elaina Ferreira de; Nepomuceno,Lívia Barreto; Pimentel,Rosiane Ferreira; Marques-da-Silva,Silvia Helena; Mesquita-da-Costa,Maurimélia.
Dermatophytosis is caused by a dermatophyte fungus that affects the stratum corneum and keratinized tissue. Dermatophyte fungus has been reported worldwide as the causative agent of dermatophytosis, but the etio-epidemiological aspects of these mycoses in the state of Pará remain unknown. The purpose of this study was to describe the etio-epidemiological profile of dermatophytosis diagnosed in patients at the Evandro Chagas Institute from May 2005 to June 2006. A total of 494 patients were admitted, and their samples were collected, submitted for direct microscopic examination using 20% KOH and cultured in Sabouraud and Mycosel medium. The identification was based in macro and microscopic characteristics. Direct examinations were positive in 13% (66/494)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dermatophytosis; Etio-epidemiology; Trichophyton; T. mentagrophytes.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200016
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Growth inhibition and morphological alterations of Trichophyton rubrum induced by essential oil from Cymbopogon winterianus Jowitt ex Bor BJM
Pereira,Fillipe de Oliveira; Wanderley,Paulo Alves; Viana,Fernando Antônio Cavalcanti; Lima,Rita Baltazar de; Sousa,Frederico Barbosa de; Lima,Edeltrudes de Oliveira.
Trichophyton rubrum is one of the most common fungi causer of dermatophytosis, mycosis that affect humans and animals around the world. Researches aiming new products with antifungal activity become necessary to overcome difficulties on treatment of these infections. Accordingly, this study aimed to investigate the antifungal activity of essential oil from Cymbopogon winterianus against the dermatophyte T. rubrum. The antifungal screening was performed by solid medium diffusion method with 16 T. rubrum strains, minimum inhibitory concentration (MIC) and minimum fungicide concentration (MFC) were determined using the microdilution method. The effects on mycelial dry weight and morphology were also observed. Screening showed essential oil in natura inhibited...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trichophyton; Essential oil; Cymbopogon.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100029
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